1. Introduction
Brown rot produced by Monilinia fructicola (Wint.) Honey (mon) is the main disease producing yield and quality losses in peaches of San Pedro (Buenos Aires, Argentina) and in other countries (Figure 1)1)(2. There is a public concern in reducing the use of synthetic fungicides, and several experiences have been conducted in order to study the effect of alternative compounds on postharvest pathogens3)(4)(5.
Essential oils, also called natural volatiles, have a complex composition containing alcohols, aldehydes, ketones, phenols, esters, ethers, and terpenes in varying proportions. Some of these compounds are recognized as “Generally regarded as safe” (gras) chemicals, stimulating interest from postharvest scientists. In vitro studies in the vapor phase have revealed that the application of the essential oils as vapors was highly effective for the control of postharvest pathogens6.
Essential oils from different species have been assayed for Monilinia fructicola control. Lemon myrtle (Backhousia citriodora) essential oil and citral were effective treatments to control mycelial growth of M. fructicola7. Other species with essential oils effective against mon are Eugenia caryophyllata, Cinnamomum zeylanium and Carum copticum8, tea tree (Melaleuca alternifolia)3)(4, savory (Satureja montana), and Thymus vulgaris9)(10.
In vitro and in vivo testing were carried out using essential oils in the vapor phase as a fumigant rather than by direct contact, as this application method seemed to be more practical for postharvest disease management11.
Fumigation with natural volatiles is promising specially in some species, like cherries, because they can be applied without wetting the fruit. The treatment of 10 mg L-1 thymol fumigation significantly reduced the incidence of brown rot, but had no effect on blue mold rot9 and produced stem discoloration.
Results from the sensory evaluation trials after fumigation indicated that lemon myrtle essential oil treatment effect on sensory value was not significant. The association between lemon myrtle essential oil treatment and intention to purchase was also not significant11.
A commercial formulate containing essential oil of Melaleuca alternifolia was effective at controlling in vitro growth of Monilinia fructicola3. This organic formulate proved to be useful in a spraying schedule, combined with fungicides and coadjutants, in order to reduce the application of synthetic products prior to peach harvest12)(13.
The effect of biofumigation, through slow-release diffusors, of thyme and savory (Satureja montana) essential oils was effective in controlling postharvest rots of peaches and nectarines. Naturally contaminated peaches and nectarines were exposed to eo vapors for 28 days at 0 ºC in sealed storage cabinets and then exposed at 20 ºC for 5 days during shelf-life in normal atmosphere, simulating retail conditions. Under low disease pressure, most treatments significantly reduced fruit rot incidence during shelf-life, while under high disease pressure only vapors of thyme essential oil at the highest concentration tested (10% v/v in the diffusor) significantly reduced the rots10.
Citrus sinensis essential oil contains limonene; this active compound showed a potent toxicity against Penicillium expansum14. Citrus industry is a big scale activity in Argentina and lemon essential oils can be obtained as a residue.
Garlic production is important in Argentina and aqueous garlic extract (ge) is obtained, commercialized and applied in different horticultural crops in order to prevent diseases and insect damage. ge contains bioactive compounds as allicin with toxic effects on several pathogens like M. fructicola15)(16, Aspergillus sp17, Botrytis cinerea, Colletotrichum sp.18. Rhizoctonia sp., Fusarium sp. and Sclerotium sp.19.
The objective of this work was the in vitro evaluation of the effect of lemon essential oil and aqueous garlic extract on Monilinia fructicola growth inhibition (inh).
2. Materials and methods
2.1 General procedures
Monilinia fructicola strains 345 and 350 obtained from peach orchards of San Pedro were grown in pda media 2% with antibiotics until they occupied all the Petri dishes. The incubation was carried out at 24 ºC and the mycelial growth was evaluated 7 days after the beginning of the experience.
All experiments were repeated twice (Rep1 and Rep2), and each treatment consisted of four replicates.
The inhibitory effect of the extracts was determined as a percentage of inhibition of radial growth of mycelium of each treatment with respect to pda control.
2.2 In vitro effect of lemon essential oil on Monilinia fructicola growth
Lemon essential oil (lo) was provided by Citrus San Miguel (Tucumán, Argentina). In Experiment 1 a mon (strain 345 and 350) colony disc of 9 mm of diameter was placed in the middle of Petri dishes containing pda 2% according to the following treatments: 1) Control, 2) lo 0.5%, 3) lo 1%, 4) lo 3% and 5) Fludioxonil (flu) 20 mL hL-1 23% suspension concentrate (sc). In Experiment 2, 1 mL of each dilution of the lemon essential oil was placed in the middle of the plate lid, and the colony of Monilinia fructicola strain 345 was located over it.
2.3 In vitro effect of aqueous garlic extract on Monilinia fructicola growth
Aqueous garlic extract (ge) was obtained processing 350 g of garlic (cultivar Colorado) in a blender with 700 mL of tap water. The volume (830 mL) of filtered extract was added with the same quantity of water. 1 mL of the treatment's suspension was placed in the middle of a cover of plates with a disc of mon (strain 345) colony of 7 mm of diameter. Treatments were: 1) Control, 2) ge 2.27%, 3) ge 5.68%, 4) ge 11.35%, and 5) Fludioxonil 20 mL hL-1 23% sc (Figure 2).
3. Results and discussion
3.1 In vitro effect of lemon essential oil on Monilinia fructicola growth
Experiment 1. Essential lemon oil added to culture media pda reduced M. fructicola growth at percentages higher than 90% in all cases and was so effective as the synthetic fungicide fludioxonil (Figure 3). Statistical differences between treatments were observed only in one repetition with strain 350 (R2= 0.95, cv= 2.30, Media= 88.52). Concentrations of 1 and 3% showed inh values of 100% for both strains and all repetitions.
The in vitro inhibitory effect of lo added to pda culture media was important, and differences between lo concentrations and chemical control were not important. lo concentrations of 1 and 3% seemed to be more promising.
Experiment 2. Highly statistical differences between treatments were observed in both repetitions (Rep1: R2= 0.78, cv=25.76, Media= 68.49; Rep 2: R2 0.91, cv= 21.39, Media= 62.07). Essential lemon oil at 3% placed under the colony of M. fructicola reduced the fungus growth at percentages of 100% and was so effective as the synthetic fungicide fludioxonil added to the culture media. Concentrations of lo of 0.5% and 1% showed lower inhibitory effects (Figures 4 and 5).
lo at 0.5% and 1% added to media was more effective reducing M. fructicola growth than applied on the Petri dish plate. According to advances in postharvest disease control, fumigation with natural volatiles is a technique that could be used for reducing synthetic fungicide application to fruits. lo at 3% should be considered for further fumigation trials with peach fruits.
3.2 In vitro effect of aqueous garlic extract on Monilinia fructicola growth
M. fructicola colonies placed over 1 mL of aqueous garlic extract were inhibited by all ge dilutions. Statistical differences (p≤0.01) were obtained only at Rep 2 (R2= 0.96, cv=14.71, Media= 44.18). ge 11.35% showed inh > 90% in both repetitions (Figures 6 and 7). The low inhibitory effect of Fludioxonil can be explained by the fact that it was applied on the Petri dish plate, and not added to the culture media.
Following these results, further investigations could be performed to evaluate postharvest fumigation with ge in order to reduce the presence of brown rot in peaches.
4. Conclusions
Several authors have obtained promising results for the in vitro and in vivo control of brown rot using natural volatiles.
This work shows that lo and ge, both natural products easily obtained in Argentina, can reach over 90% of in vitro mycelial growth inhibition of Monilinia fructicola.
Following these results, lo and ge could be evaluated in future trials as postharvest treatments to peach fruits for Monilinia fructicola control.
Methods of application should be assayed, and further assays developed, in order to evaluate these alternative compounds to synthetic fungicides.